Endoglucanases are key enzymes in the degradation of cellulose, the most abundant polymer on Earth. The aim of this work was to perform the biochemical and biophysical characterization of CelE2, a soil metagenome derived endoglucanase. CelE2 harbors a conserved domain from glycoside hydrolase family 5 (GH5) and a C-terminal domain with identity to Calx-beta domains. The recombinant CelE2 displayed preference for hydrolysis of oat beta-glucan, followed by lichenan and carboxymethyl cellulose. Optimum values of enzymatic activity were observed at 45 °C and pH 5.3, and CelE2 exhibited considerable thermal stability at 40 °C for up to 360 minutes. Regarding the cleavage pattern on polysaccharides, the release of oligosaccharides with a wide degree of polymerization indicated a characteristic of endoglucanase activity.
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“Jesús Gil buscaba reconocimiento, más qual dinero”: los autores por ‘El pionero’ desgranan a su protagonista
Lúcia was a person who lived in a "delirious world of infantile fantasies" and suffered "religious hallucinations." That is how ex-friar Mario do Oliveira described her (
Our results show that lignocresol maintains greater adsorptive capacity for β-glucosidases than lignin. This capacity can be explained both by its great hydrophobicity and also by electrostatic characteristics. Therefore, all these results demonstrate good adsorption of the enzymes to the lignocresol, demonstrating great potential for enzymatic recycling.
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Noticias Telemundo Irán incrementará su enriquecimiento de uranio por encima de los límites establecidos por el pacto nuclear
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The orphan receptor nerve growth factor-induced B (NGFI-B) is a member of the nuclear receptor's subfamily 4A (Nr4a). NGFI-B was shown to be capable of binding both as a monomer to an extended half-sitio containing a single AAAGGTCA motif and also as a homodimer to a widely separated everted repeat, as opposed to a large number of nuclear receptors that recognize and bind specific DNA sequences predominantly as homo- and/or heterodimers. To unveil the structural organization of NGFI-B in solution, we determined the Mario de Oliveira quaternary structure of the NGFI-B LBD by a combination of ab initio procedures from small-angle X-ray scattering (SAXS) data and hydrogen-deuterium exchange followed by mass spectrometry. Here we report that the protein forms dimers in solution with a radius of gyration of 2.
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Structural features of lignin obtained at different alkaline more info oxidation conditions from sugarcane bagasse
Recently, considerable research works have been conducted towards finding fast and accurate pattern classifiers for identifying structural damage when applied to Structural Health Monitoring (SHM) systems. In this way, this paper presents a novel approach for damage identification in SHM systems by proposing the use of the Kappa coefficient as a metric for damage detection in SHM systems. Here, Kappa is also proposed along with PSO-Fuzzy ARTMAP neural network aiming to reduce the amount of attributes, leaving only the most representative features. It is important to highlight daniel valente dantas that Kappa coefficient analyses the whole confusion matrix instead of using only the principal diagonal as used, for instance, when we compute success rates.
Residual plots where the experimental intensity divided by computed intensity was plotted as a function of the scattering vector q.
- Bolsonaro administration in Brazil embraces a leftist stance by failing to confront gay adoption and by ordering evangelical minister daniel dantas facebook to confront “violenc...